• Western blot analysis of extracts of various cell lines, using Peroxiredoxin 1/PAG Rabbit monoclonal antibody (STJ11102205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRDX1 antibody (1-100) [S5MR] (STJ11102205)

SKU:
STJ11102205

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PRDX1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDX1
Gene ID: 5052
Uniprot ID: PRDX1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Peroxiredoxin 1/PAG (Q06830).
Immunogen Sequence: MSSGNAKIGHPAPNFKATAV MPDGQFKDISLSDYKGKYVV FFFYPLDFTFVCPTEIIAFS DRAEEFKKLNCQVIGASVDS HFCHLAWVNTPKKQGGLGPM
Post Translational Modifications Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.
Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Protein Name Peroxiredoxin-1
Natural Killer Cell-Enhancing Factor A
Nkef-A
Proliferation-Associated Gene Protein
Pag
Thioredoxin Peroxidase 2
Thioredoxin-Dependent Peroxide Reductase 2
Thioredoxin-Dependent Peroxiredoxin 1
Database Links Reactome: R-HSA-3299685
Reactome: R-HSA-5628897
Reactome: R-HSA-8862803
Reactome: R-HSA-9818027
Cellular Localisation Cytoplasm
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Peroxiredoxin-1 antibody
Anti-Natural Killer Cell-Enhancing Factor A antibody
Anti-Nkef-A antibody
Anti-Proliferation-Associated Gene Protein antibody
Anti-Pag antibody
Anti-Thioredoxin Peroxidase 2 antibody
Anti-Thioredoxin-Dependent Peroxide Reductase 2 antibody
Anti-Thioredoxin-Dependent Peroxiredoxin 1 antibody
Anti-PRDX1 antibody
Anti-PAGA antibody
Anti-PAGB antibody
Anti-TDPX2 antibody

Information sourced from Uniprot.org

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