Anti-PRDM16/MEL1 antibody (C-Term) (STJ70230)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PRDM16/MEL1 (C-Term) is suitable for use in ELISA, Flow Cytometry and ChImmunoprecipitation research applications. |
| Applications | Pep-ELISA/FC/ChIP |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.FC:Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. ChIP: This product has been successfully used in ChIP on Mouse: Hondares E et al. (2011) |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PRDM16 |
| Gene ID | 63976 |
| Uniprot ID | PRD16_HUMAN |
| Accession Number | NP_071397.3, NP_955533.2 |
| Immunogen Region | C-Term |
| Immunogen Sequence | TSESGAFHPINHL |
| Specificity | This antibody is expected to recognise both reported isoforms. |
Additional Info
| Function | Binds DNA and functions as a transcriptional regulator. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions as a repressor of TGF-beta signaling. Isoform 4: Binds DNA and functions as a transcriptional regulator. Functions as a repressor of TGF-beta signaling. May regulate granulocyte differentiation. |
| Protein Name | Histone-Lysine N-Methyltransferase Prdm16Pr Domain Zinc Finger Protein 16Pr Domain-Containing Protein 16Transcription Factor Mel1Mds1/Evi1-Like Gene 1 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-9844594 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Prdm16 antibodyAnti-Pr Domain Zinc Finger Protein 16 antibodyAnti-Pr Domain-Containing Protein 16 antibodyAnti-Transcription Factor Mel1 antibodyAnti-Mds1/Evi1-Like Gene 1 antibodyAnti-PRDM16 antibodyAnti-KIAA1675 antibodyAnti-MEL1 antibodyAnti-PFM13 antibody |
Information sourced from Uniprot.org