Anti-PRDM16 antibody (C-Term) (STJA0010358)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human C-ternal PRD16
STJA0010358
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General Information

Short DescriptionRabbit polyclonal anti-Histone-lysine N-methyltransferase PRDM16 (C-Term) for use in IHC and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
IHC 1:50-200
FormulationLiquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPRDM16
Gene ID63976
Uniprot IDPRD16_HUMAN
ImmunogenSynthesized peptide derived from human C-ternal PRD16
Immunogen RegionC-Term
SpecificityThis antibody detects endogenous levels of PRD16 at Human, Mouse

Additional Info

Function Binds DNA and functions as a transcriptional regulator. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions as a repressor of TGF-beta signaling. Isoform 4: Binds DNA and functions as a transcriptional regulator. Functions as a repressor of TGF-beta signaling. May regulate granulocyte differentiation.
Protein Name Histone-Lysine N-Methyltransferase Prdm16
Pr Domain Zinc Finger Protein 16
Pr Domain-Containing Protein 16
Transcription Factor Mel1
Mds1/Evi1-Like Gene 1
Database Links Reactome: R-HSA-3214841
Reactome: R-HSA-9844594
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Prdm16 antibody
Anti-Pr Domain Zinc Finger Protein 16 antibody
Anti-Pr Domain-Containing Protein 16 antibody
Anti-Transcription Factor Mel1 antibody
Anti-Mds1/Evi1-Like Gene 1 antibody
Anti-PRDM16 antibody
Anti-KIAA1675 antibody
Anti-MEL1 antibody
Anti-PFM13 antibody

Information sourced from Uniprot.org

Citations

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