• Western blot analysis of lysates from U-87MG cells, using PRDM12 Rabbit polyclonal antibody (STJ119786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRDM12 antibody (100-330) (STJ119786)

SKU:
STJ119786

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDM12 (100-330) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDM12
Gene ID: 59335
Uniprot ID: PRD12_HUMAN
Immunogen Region: 100-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human PRDM12 (NP_067632.2).
Immunogen Sequence: LGIFSKTWIKAGTEMGPFTG RVIAPEHVDICKNNNLMWEV FNEDGTVRYFIDASQEDHRS WMTYIKCARNEQEQNLEVVQ IGTSIFYKAIEMIPPDQELL VWYGNSHNTFLGIPGVPGLE EDQKKNKHEDFHPADSAAGP AGRMRCVICHRGFNSRSNLR SHMRIHTLDKPFVCRFCNRR FSQSSTLRNHVRLHTGERPY KCQVCQSAYSQLAGLRAHQK SARHRPPSTAL
Tissue Specificity Not found in adult tissues except in dorsal root ganglia.
Function Involved in the positive regulation of histone H3-K9 dimethylation.
Protein Name Pr Domain Zinc Finger Protein 12
Pr Domain-Containing Protein 12
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 12 antibody
Anti-Pr Domain-Containing Protein 12 antibody
Anti-PRDM12 antibody
Anti-PFM9 antibody

Information sourced from Uniprot.org

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