Anti-PRAME antibody (100-509) [ZR383] (STJ180621)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant fragment (around aa100-509) of human PRAME protein
General Information
| Short Description | Rabbit monoclonal PRAME (100-509) antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZR383 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | 1:100-200 |
| Formulation | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Storage Instruction | Store at 2β8Β°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate labβstandardized diluent. Stability after dilution: 7 days at 24Β°C, 3 months at 2β8Β°C, 6months at β20Β°C. |
Target Information
| Gene Symbol | PRAME |
| Gene ID | 23532 |
| Uniprot ID | PRAME_HUMAN |
| Immunogen | Recombinant fragment (around aa100-509) of human PRAME protein |
| Immunogen Region | 100-509 |
| Specificity | Positive Control: Testis or melanoma |
Additional Info
| Tissue Specificity | Expressed in testis. Detected in samples of kidney, brain and skin. |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation. Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. |
| Protein Name | Melanoma Antigen Preferentially Expressed In TumorsOpa-Interacting Protein 4Oip-4Preferentially Expressed Antigen Of Melanoma |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasmGolgi ApparatusCell MembraneAssociates With ChromatinSpecifically Enriched At Transcriptionally Active Promoters That Are Also Bound By Nuclear Transcription Factor Y (Composed Of NfyaNfyb And Nfyc) And At EnhancersRecruited To The Golgi Apparatus In Response To Interferon Gamma (Ifng) Treatment |
| Alternative Antibody Names | Anti-Melanoma Antigen Preferentially Expressed In Tumors antibodyAnti-Opa-Interacting Protein 4 antibodyAnti-Oip-4 antibodyAnti-Preferentially Expressed Antigen Of Melanoma antibodyAnti-PRAME antibodyAnti-MAPE antibodyAnti-OIP4 antibody |
Information sourced from Uniprot.org