• Western blot analysis of extracts of various cell lines, using PQBP1 antibody (STJ25098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PQBP1 antibody (1-265) (STJ25098)

SKU:
STJ25098

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PQBP1 (1-265) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PQBP1
Gene ID: 10084
Uniprot ID: PQBP1_HUMAN
Immunogen Region: 1-265
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human PQBP1 (NP_005701.1).
Immunogen Sequence: MPLPVALQTRLAKRGILKHL EPEPEEEIIAEDYDDDPVDY EATRLEGLPPSWYKVFDPSC GLPYYWNADTDLVSWLSPHD PNSVVTKSAKKLRSSNADAE EKLDRSHDKSDRGHDKSDRS HEKLDRGHDKSDRGHDKSDR DRERGYDKVDRERERDRERD RDRGYDKADREEGKERRHHR REELAPYPKSKKAVSRKDEE LDPMDPSSYSDAPRGTWSTG LPKRNEAKTGADTTAAGPL
Tissue Specificity Widely expressed with high level in heart, skeletal muscle, pancreas, spleen, thymus, prostate, ovary, small intestine and peripheral blood leukocytes.
Function Intrinsically disordered protein that acts as a scaffold, and which is involved in different processes, such as pre-mRNA splicing, transcription regulation, innate immunity and neuron development. Interacts with splicing-related factors via the intrinsically disordered region and regulates alternative splicing of target pre-mRNA species. May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit. Involved in the assembly of cytoplasmic stress granule, possibly by participating in the transport of neuronal RNA granules. Also acts as an innate immune sensor of infection by retroviruses, such as HIV, by detecting the presence of reverse-transcribed DNA in the cytosol. Directly binds retroviral reverse-transcribed DNA in the cytosol and interacts with CGAS, leading to activate the cGAS-STING signaling pathway, triggering type-I interferon production.
Protein Name Polyglutamine-Binding Protein 1
Pqbp-1
38 Kda Nuclear Protein Containing A Ww Domain
Npw38
Polyglutamine Tract-Binding Protein 1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasmic Granule
Colocalizes With Srsf2 In Nuclear Speckles
Colocalized With Pou3f2
Colocalized With Atxn1 In Nuclear Inclusion Bodies
Localizes To Cytoplasmic Stress Granules
Alternative Antibody Names Anti-Polyglutamine-Binding Protein 1 antibody
Anti-Pqbp-1 antibody
Anti-38 Kda Nuclear Protein Containing A Ww Domain antibody
Anti-Npw38 antibody
Anti-Polyglutamine Tract-Binding Protein 1 antibody
Anti-PQBP1 antibody
Anti-NPW38 antibody
Anti-JM26 antibody

Information sourced from Uniprot.org

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