Anti-PPP4R2 antibody (171-220 aa) (STJ95199)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PPP4R2 at the amino acid range 171-220
General Information
| Short Description | Rabbit polyclonal anti-Serine/threonine-protein phosphatase 4 regulatory subunit 2 (171-220 aa) for use in WB, IHC, IF and ELISA in Human and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PPP4R2 |
| Gene ID | 151987 |
| Uniprot ID | PP4R2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PPP4R2 at the amino acid range 171-220 |
| Immunogen Region | 171-220 aa |
| Specificity | PP4R2 Polyclonal Antibody detects endogenous levels of PP4R2 protein. |
Additional Info
| Function | Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair. |
| Protein Name | Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 2 |
| Database Links | Reactome: R-HSA-5693607 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleusIonizing Radiation Induces Relocalization To Nuclear Foci And Colocalization With Rpa2 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 2 antibodyAnti-PPP4R2 antibodyAnti-SBBI57 antibody |
Information sourced from Uniprot.org