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Anti-PPP2R5D antibody (C-Term) (STJ70081)

STJ70081 (0. 1µg/ml) staining of A431 (A) , Jurkat (B) and HepG2 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
STJ70081 (0. 1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
STJ70081 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
STJ70081 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PPP2R5D antibody (C-Term) (STJ70081)

SKU:

STJ70081-100

£303.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Goat
Applications:	Pep-ELISA/WB/IF/FC
Reactivity:	Human/Mouse/Rat/Dog/Pig/Cow
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Goat polyclonal antibody anti-PPP2R5D (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification:	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration:	0.5 mg/mL
Dilution Range:	IF-Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. 10µg/ml ELISA-antibody detection limit dilution 1:20000.
Storage Instruction:	Store at-20°C on receipt and minimise freeze-thaw cycles.

Gene Symbol:	PPP2R5D
Gene ID:	5528
Uniprot ID:	2A5D_HUMAN
Immunogen Region:	C-Term
Accession Number:	NP_006236.1; NP_851307.1; NP_851308.1; NP_001257405.1
Specificity:	This antibody is expected to recognise reported isoforms (NP_006236.1 (iso 1) ; NP_851307.1; (iso 2) NP_851308.1) (iso 3) and NP_001257405.1 (iso 4).
Immunogen Sequence:	KRAEEFLTASQEAL

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Protein Name	Serine/Threonine-Protein Phosphatase 2a 56 Kda Regulatory Subunit Delta Isoform Pp2a B Subunit Isoform B'-Delta Pp2a B Subunit Isoform B56-Delta Pp2a B Subunit Isoform Pr61-Delta Pp2a B Subunit Isoform R5-Delta
Database Links	Reactome: R-HSA-141444 Reactome: R-HSA-163685 Reactome: R-HSA-163767 Reactome: R-HSA-180024 Reactome: R-HSA-195253 Reactome: R-HSA-196299 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-389513 Reactome: R-HSA-432142 Reactome: R-HSA-4641262 Reactome: R-HSA-5339716 Reactome: R-HSA-5358747 Reactome: R-HSA-5358749 Reactome: R-HSA-5358751 Reactome: R-HSA-5358752 Reactome: R-HSA-5467337 Reactome: R-HSA-5467340 Reactome: R-HSA-5467348 Reactome: R-HSA-5663220 Reactome: R-HSA-5673000 Reactome: R-HSA-5675221 Reactome: R-HSA-6811558 Reactome: R-HSA-68877 Reactome: R-HSA-9634600 Reactome: R-HSA-9648025
Cellular Localisation	Cytoplasm Nucleus Nuclear In Interphase Nuclear During Mitosis
Alternative Antibody Names	Anti-Serine/Threonine-Protein Phosphatase 2a 56 Kda Regulatory Subunit Delta Isoform antibody Anti-Pp2a B Subunit Isoform B'-Delta antibody Anti-Pp2a B Subunit Isoform B56-Delta antibody Anti-Pp2a B Subunit Isoform Pr61-Delta antibody Anti-Pp2a B Subunit Isoform R5-Delta antibody Anti-PPP2R5D antibody

Information sourced from Uniprot.org

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