• Western blot analysis of extracts of various cell lines, using PPP1R8 antibody (STJ28784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat brain using PPP1R8 Antibody (STJ28784) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human breast cancer using PPP1R8 Antibody (STJ28784) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using PPP1R8 Antibody (STJ28784) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of MCF7 cells using PPP1R8 antibody (STJ28784).

Anti-PPP1R8 antibody (1-209) (STJ28784)

SKU:
STJ28784

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1R8 (1-209) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R8
Gene ID: 5511
Uniprot ID: PP1R8_HUMAN
Immunogen Region: 1-209
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human PPP1R8 (NP_612568.1).
Immunogen Sequence: MGGEDDELKGLLGLPEEETE LDNLTEFNTAHNKRISTLTI EEGNLDIQRPKRKRKNSRVT FSEDDEIINPEDVDPSVGRF RNMVQTAVVPVKKKRVEGPG SLGLEESGSRRMQNFAFSGG LYGGLPPTHSEAGSQPHGIH GTALIGGLPMPYPNLAPDVD LTPVVPSAVNMNPAPNPAVY NPEAVNEPKKKKYAKEAWPG KKPTPSLLI
Tissue Specificity Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
Post Translational Modifications May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
Function Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
Protein Name Nuclear Inhibitor Of Protein Phosphatase 1
Nipp-1
Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay
Ard-1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Primarily
But Not Exclusively
Nuclear
Isoform Gamma: Cytoplasm
Found Mainly In The Cytoplasm
Alternative Antibody Names Anti-Nuclear Inhibitor Of Protein Phosphatase 1 antibody
Anti-Nipp-1 antibody
Anti-Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay antibody
Anti-Ard-1 antibody
Anti-PPP1R8 antibody
Anti-ARD1 antibody
Anti-NIPP1 antibody

Information sourced from Uniprot.org

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