• Western blot analysis of HeLa, using GMNN antibody (STJ27269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GMNN rabbit polyclonal antibody (STJ27269) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GMNN rabbit polyclonal antibody (STJ27269) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using GMNN rabbit polyclonal antibody (STJ27269) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of MCF7 cells using GMNN rabbit polyclonal antibody (STJ27269) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GMNN antibody (1-209) (STJ27269)

SKU:
STJ27269

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GMNN (1-209) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GMNN
Gene ID: 51053
Uniprot ID: GEMI_HUMAN
Immunogen Region: 1-209
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1).
Immunogen Sequence: MNPSMKQKQEEIKENIKNSS VPRRTLKMIQPSASGSLVGR ENELSAGLSKRKHRNDHLTS TTSSPGVIVPESSENKNLGG VTQESFDLMIKENPSSQYWK EVAEKRRKALYEALKENEKL HKEIEQKDNEIARLKKENKE LAEVAEHVQYMAELIERLNG EPLDNFESLDNQEFDSEEET VEDSLVEDSEIGTCAEGTVS SSTDAKPCI
Post Translational Modifications Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.
Function Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing. Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control.
Protein Name Geminin
Database Links Reactome: R-HSA-68867
Reactome: R-HSA-68962
Reactome: R-HSA-69052
Cellular Localisation Cytoplasm
Nucleus
Mainly Cytoplasmic But Can Be Relocalized To The Nucleus
Alternative Antibody Names Anti-Geminin antibody
Anti-GMNN antibody

Information sourced from Uniprot.org

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