Anti-GMNN antibody (1-209) (STJ27269)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GMNN (1-209) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GMNN |
| Gene ID | 51053 |
| Uniprot ID | GEMI_HUMAN |
| Immunogen Region | 1-209 |
| Immunogen Sequence | MNPSMKQKQEEIKENIKNSS VPRRTLKMIQPSASGSLVGR ENELSAGLSKRKHRNDHLTS TTSSPGVIVPESSENKNLGG VTQESFDLMIKENPSSQYWK EVAEKRRKALYEALKENEKL HKEIEQKDNEIARLKKENKE LAEVAEHVQYMAELIERLNG EPLDNFESLDNQEFDSEEET VEDSLVEDSEIGTCAEGTVS SSTDAKPCI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1). |
Additional Info
| Post Translational Modifications | Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity. |
| Function | Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing. Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control. |
| Protein Name | Geminin |
| Database Links | Reactome: R-HSA-68867Reactome: R-HSA-68962Reactome: R-HSA-69052 |
| Cellular Localisation | CytoplasmNucleusMainly Cytoplasmic But Can Be Relocalized To The Nucleus |
| Alternative Antibody Names | Anti-Geminin antibodyAnti-GMNN antibody |
Information sourced from Uniprot.org