Anti-NIPP1/PPP1R8 antibody (C-Term) (STJ70400)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-NIPP1/PPP1R8 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PPP1R8 |
| Gene ID | 5511 |
| Uniprot ID | PP1R8_HUMAN |
| Accession Number | NP_002704.1; NP_054829.2; NP_612568.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | EAWPGKKPTPSLLI |
| Specificity | This antibody is expected to recognise all three human isoforms of this protein. |
Additional Info
| Post Translational Modifications | May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA. |
| Function | Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. |
| Protein Name | Nuclear Inhibitor Of Protein Phosphatase 1Nipp-1Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna DecayArd-1 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleus SpecklePrimarilyBut Not ExclusivelyNuclearIsoform Gamma: CytoplasmFound Mainly In The Cytoplasm |
| Alternative Antibody Names | Anti-Nuclear Inhibitor Of Protein Phosphatase 1 antibodyAnti-Nipp-1 antibodyAnti-Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay antibodyAnti-Ard-1 antibodyAnti-PPP1R8 antibodyAnti-ARD1 antibodyAnti-NIPP1 antibody |
Information sourced from Uniprot.org