• STJ70400 (1µg/ml) staining of HeLa (A) , HepG2 (B) , Jurkat (C) and NIH3T3 (D) nuclear lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-NIPP1/PPP1R8 antibody (C-Term) (STJ70400)

SKU:
STJ70400-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NIPP1/PPP1R8 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-2.5-3.8µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PPP1R8
Gene ID: 5511
Uniprot ID: PP1R8_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002704.1; NP_054829.2; NP_612568.1
Specificity: This antibody is expected to recognise all three human isoforms of this protein.
Immunogen Sequence: EAWPGKKPTPSLLI
Post Translational Modifications May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
Function Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
Protein Name Nuclear Inhibitor Of Protein Phosphatase 1
Nipp-1
Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay
Ard-1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Primarily
But Not Exclusively
Nuclear
Isoform Gamma: Cytoplasm
Found Mainly In The Cytoplasm
Alternative Antibody Names Anti-Nuclear Inhibitor Of Protein Phosphatase 1 antibody
Anti-Nipp-1 antibody
Anti-Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay antibody
Anti-Ard-1 antibody
Anti-PPP1R8 antibody
Anti-ARD1 antibody
Anti-NIPP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance