Western blot analysis of various lysates using PPM1F Rabbit polyclonal antibody (STJ117558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PPM1F antibody (1-190) (STJ117558)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ117558
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General Information

Short DescriptionRabbit polyclonal PPM1F (1-190) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPPM1F
Gene ID9647
Uniprot IDPPM1F_HUMAN
Immunogen Region1-190
Immunogen SequenceMSSGAPQKSSPMASGAEETP GFLDTLLQDFPALLNPEDPL PWKAPGTVLSQEEVEGELAE LAMGFLGSRKAPPPLAAALA HEAVSQLLQTDLSEFRKLPR EEEEEEEDDDEEEKAPVTLL DAQSLAQSFFNRLWEVAGQW QKQVPLAARASQRQWLVSIH AIRNTRRKMEDRHVSLPSFN QLFGLSDPVN
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_055449.1).

Additional Info

Function Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
Protein Name Protein Phosphatase 1f
Ca(2+/Calmodulin-Dependent Protein Kinase Phosphatase
Cam-Kinase Phosphatase
Camkpase
Partner Of Pix 2
Protein Fem-2 Homolog
Hfem-2
Database Links Reactome: R-HSA-9617324
Cellular Localisation
Alternative Antibody Names Anti-Protein Phosphatase 1f antibody
Anti-Ca(2+/Calmodulin-Dependent Protein Kinase Phosphatase antibody
Anti-Cam-Kinase Phosphatase antibody
Anti-Camkpase antibody
Anti-Partner Of Pix 2 antibody
Anti-Protein Fem-2 Homolog antibody
Anti-Hfem-2 antibody
Anti-PPM1F antibody
Anti-KIAA0015 antibody
Anti-POPX2 antibody

Information sourced from Uniprot.org

Citations

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