• Immunofluorescence analysis of NIH-3T3 cells using PPM1A Rabbit polyclonal antibody (STJ28782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PPM1A Rabbit polyclonal antibody (STJ28782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PPM1A Rabbit polyclonal antibody (STJ28782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PPM1A antibody (STJ28782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PPM1A antibody (161-370) (STJ28782)

SKU:
STJ28782

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPM1A (161-370) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPM1A
Gene ID: 5494
Uniprot ID: PPM1A_HUMAN
Immunogen Region: 161-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 161-370 of human PPM1A (NP_005029.1).
Immunogen Sequence: ILENVEVKGEKPAKLCVIAE CGELKEGDDGGIFPKDGSGD SHPDFPEDADIDLKDVDKIL LITEDLKNIGNTFFKSQNWE MAIKKYAEVLRYVDSSKAVI ETADRAKLQPIALSCVLNIG ACKLKMSNWQGAIDSCLEAL ELDPSNTKALYRRAQGWQGL KEYDQALADLKKAQGIAPED KAIQAELLKVKQKIKAQKDK EKAVYAKMFA
Post Translational Modifications N-myristoylation is essential for the recognition of its substrates for dephosphorylation.
Function Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
Protein Name Protein Phosphatase 1a
Protein Phosphatase 2c Isoform Alpha
Pp2c-Alpha
Protein Phosphatase Ia
Database Links Reactome: R-HSA-2173795
Reactome: R-HSA-380972
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Membrane
Lipid-Anchor
Weakly Associates At The Membrane And N-Myristoylation Mediates The Membrane Localization
Alternative Antibody Names Anti-Protein Phosphatase 1a antibody
Anti-Protein Phosphatase 2c Isoform Alpha antibody
Anti-Pp2c-Alpha antibody
Anti-Protein Phosphatase Ia antibody
Anti-PPM1A antibody
Anti-PPPM1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance