• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g PPIE antibody (STJ29141). Western blot was performed from the immunoprecipitate using PPIE antibody (STJ29141) at a dilution of 1:1000.
  • Western blot analysis of various lysates using PPIE Rabbit polyclonal antibody (STJ29141) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPIE antibody (1-301) (STJ29141)

SKU:
STJ29141

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPIE (1-301) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIE
Gene ID: 10450
Uniprot ID: PPIE_HUMAN
Immunogen Region: 1-301
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human PPIE (NP_006103.1).
Immunogen Sequence: MATTKRVLYVGGLAEEVDDK VLHAAFIPFGDITDIQIPLD YETEKHRGFAFVEFELAEDA AAAIDNMNESELFGRTIRVN LAKPMRIKEGSSRPVWSDDD WLKKFSGKTLEENKEEEGSE PPKAETQEGEPIAKKARSNP QVYMDIKIGNKPAGRIQMLL RSDVVPMTAENFRCLCTHEK GFGFKGSSFHRIIPQFMCQG GDFTNHNGTGGKSIYGKKFD DENFILKHTGPGLLSMANS
Tissue Specificity Found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Function Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity.this requires proline isomerase activity.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase E
Ppiase E
Cyclophilin E
Cyclophilin-33
Rotamase E
Database Links Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6798695
Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase E antibody
Anti-Ppiase E antibody
Anti-Cyclophilin E antibody
Anti-Cyclophilin-33 antibody
Anti-Rotamase E antibody
Anti-PPIE antibody
Anti-CYP33 antibody

Information sourced from Uniprot.org

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