• Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Cyclophilin B monoclonal antibody (2B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Liver Tissue, 4) 3T3 with Cyclophilin B Mouse mAb diluted at 1:2, 000.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Cyclophilin B monoclonal antibody (2B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Cyclophilin B monoclonal antibody (2B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-spleen tissue. 1, Cyclophilin B monoclonal antibody (2B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-lung tissue. 1, Cyclophilin B monoclonal antibody (2B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, Calmodulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-PPIB antibody [2B10] (STJ97530)

SKU:
STJ97530

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IF/WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Peptidyl-prolyl cis-trans isomerase B is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 2B10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:1000-2000
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIB
Gene ID: 5479
Uniprot ID: PPIB_HUMAN
Specificity: Cyclophilin B protein detects endogenous levels of Cyclophilin B
Immunogen: Synthetic Peptide of Cyclophilin B
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase B
Ppiase B
Cyp-S1
Cyclophilin B
Rotamase B
S-Cyclophilin
Scylp
Database Links Reactome: R-HSA-1650814
Reactome: R-HSA-9692916
Cellular Localisation Virion
(Microbial Infection)
Endoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase B antibody
Anti-Ppiase B antibody
Anti-Cyp-S1 antibody
Anti-Cyclophilin B antibody
Anti-Rotamase B antibody
Anti-S-Cyclophilin antibody
Anti-Scylp antibody
Anti-PPIB antibody
Anti-CYPB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance