• Immunofluorescence analysis of HeLa cells using PPAT Polyclonal Antibody (STJ28781) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of Rat spleen, using PPAT Rabbit polyclonal antibody (STJ28781) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PPAT antibody (1-280) (STJ28781)

SKU:
STJ28781

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPAT (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPAT
Gene ID: 5471
Uniprot ID: PUR1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3).
Immunogen Sequence: MELEELGIREECGVFGCIAS GEWPTQLDVPHVITLGLVGL QHRGQESAGIVTSDGSSVPT FKSHKGMGLVNHVFTEDNLK KLYVSNLGIGHTRYATTGKC ELENCQPFVVETLHGKIAVA HNGELVNAARLRKKLLRHGI GLSTSSDSEMITQLLAYTPP QEQDDTPDWVARIKNLMKEA PTAYSLLIMHRDVIYAVRDP YGNRPLCIGRLIPVSDINDK EKKTSETEGWVVSSESCSF
Tissue Specificity Ubiquitously expressed.
Function Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.
Protein Name Amidophosphoribosyltransferase
Atase
Glutamine Phosphoribosylpyrophosphate Amidotransferase
Gpat
Database Links Reactome: R-HSA-73817
Alternative Antibody Names Anti-Amidophosphoribosyltransferase antibody
Anti-Atase antibody
Anti-Glutamine Phosphoribosylpyrophosphate Amidotransferase antibody
Anti-Gpat antibody
Anti-PPAT antibody
Anti-GPAT antibody

Information sourced from Uniprot.org

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