| Host: |
Mouse |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Peroxisome proliferator-activated receptor gamma coactivator 1-alpha is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
1G8 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PPARGC1A |
| Gene ID: |
10891 |
| Uniprot ID: |
PRGC1_HUMAN |
| Specificity: |
PGC-1 Alpha Monoclonal Antibody detects endogenous levels of PGC-1 Alpha protein. |
| Immunogen: |
Purified recombinant fragment of human PGC-1 Alpha expressed in E. Coli. |
| Post Translational Modifications | Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by KAT2A/GCN5 under conditions of high nutrients, leading to inactivation of PPARGC1A. Deacetylated by SIRT1 in low nutrients/high NAD conditions, leading to its activation. Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. |
| Function | Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. |
| Protein Name | Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-AlphaPgc-1-AlphaPpar-Gamma Coactivator 1-AlphaPpargc-1-AlphaLigand Effect Modulator 6 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2151201Reactome: R-HSA-2151209Reactome: R-HSA-381340Reactome: R-HSA-3899300Reactome: R-HSA-400253Reactome: R-HSA-8939902Reactome: R-HSA-9615017Reactome: R-HSA-9707616 |
| Cellular Localisation | Isoform 1: NucleusNucleusPml BodyIsoform B4: NucleusIsoform B4-8a: CytoplasmIsoform B5: NucleusIsoform 9: Nucleus |
| Alternative Antibody Names | Anti-Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha antibodyAnti-Pgc-1-Alpha antibodyAnti-Ppar-Gamma Coactivator 1-Alpha antibodyAnti-Ppargc-1-Alpha antibodyAnti-Ligand Effect Modulator 6 antibodyAnti-PPARGC1A antibodyAnti-LEM6 antibodyAnti-PGC1 antibodyAnti-PGC1A antibodyAnti-PPARGC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
