Anti-PPAR delta-Isoform 1 antibody (C-Term) (STJ70591)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PPAR delta-Isoform 1 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9kDa according to NP_006229.1). Recommended concentration: 1-3ยตg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PPARD |
| Gene ID | 5467 |
| Uniprot ID | PPARD_HUMAN |
| Accession Number | NP_006229.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | CHPLLQEIYKDMY |
| Specificity | This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus. |
Additional Info
| Post Translational Modifications | 'Lys-48'-linked polyubiquitinated.leading to proteasomal degradation. Deubiquitinated and stabilized by OTUD3. |
| Function | Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. |
| Protein Name | Peroxisome Proliferator-Activated Receptor DeltaPpar-DeltaNuciNuclear Hormone Receptor 1Nuc1Nuclear Receptor Subfamily 1 Group C Member 2Peroxisome Proliferator-Activated Receptor BetaPpar-Beta |
| Database Links | Reactome: R-HSA-200425Reactome: R-HSA-383280Reactome: R-HSA-5362517 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Peroxisome Proliferator-Activated Receptor Delta antibodyAnti-Ppar-Delta antibodyAnti-Nuci antibodyAnti-Nuclear Hormone Receptor 1 antibodyAnti-Nuc1 antibodyAnti-Nuclear Receptor Subfamily 1 Group C Member 2 antibodyAnti-Peroxisome Proliferator-Activated Receptor Beta antibodyAnti-Ppar-Beta antibodyAnti-PPARD antibodyAnti-NR1C2 antibodyAnti-PPARB antibody |
Information sourced from Uniprot.org