• Immunofluorescence analysis of A549 cells using PPAN antibody (STJ29514).
  • Immunohistochemistry analysis of paraffin-embedded human stomach using PPAN antibody (STJ29514) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using PPAN antibody (STJ29514) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PPAN Antibody (STJ29514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PPAN antibody (1-300) (STJ29514)

SKU:
STJ29514

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPAN (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPAN
Gene ID: 56342/692312
Uniprot ID: SSF1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3).
Immunogen Sequence: MGQSGRSRHQKRARAQAQLR NLEAYAANPHSFVFTRGCTG RNIRQLSLDVRRVMEPLTAS RLQVRKKNSLKDCVAVAGPL GVTHFLILSKTETNVYFKLM RLPGGPTLTFQVKKYSLVRD VVSSLRRHRMHEQQFAHPPL LVLNSFGPHGMHVKLMATMF QNLFPSINVHKVNLNTIKRC LLIDYNPDSQELDFRHYSIK VVPVGASRGMKKLLQEKFPN MSRLQDISELLATGAGLSE
Tissue Specificity Widely expressed.
Function May have a role in cell growth.
Protein Name Suppressor Of Swi4 1 Homolog
Ssf-1
Brix Domain-Containing Protein 3
Peter Pan Homolog
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Suppressor Of Swi4 1 Homolog antibody
Anti-Ssf-1 antibody
Anti-Brix Domain-Containing Protein 3 antibody
Anti-Peter Pan Homolog antibody
Anti-PPAN antibody
Anti-BXDC3 antibody
Anti-SSF1 antibody

Information sourced from Uniprot.org

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