Anti-POU4F2 antibody (Internal) (STJ72236)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-POU4F2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 43.1kDa according to Human NP_004566.2, ). This band was successfully blocked by incubation with the |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | POU4F2 |
| Gene ID | 5458 |
| Uniprot ID | PO4F2_HUMAN |
| Accession Number | NP_004566.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | HGLPSHMGCMSDVD |
| Specificity | This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459) |
Additional Info
| Function | Tissue-specific DNA-binding transcription factor involved in the development and differentiation of target cells. Functions either as activator or repressor modulating the rate of target gene transcription through RNA polymerase II enzyme in a promoter-dependent manner. Binds to the consensus octamer motif 5'-ATA/TAT/ATA/TA-3' of promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Binds to an octamer site to form a ternary complex with ISL1.cooperates positively with ISL1 and ISL2 to potentiate transcriptional activation of RGC target genes being involved in RGC fate commitment in the developing retina and RGC axon formation and pathfinding. Inhibits DLX1 and DLX2 transcriptional activities preventing DLX1- and DLX2-mediated ability to promote amacrine cell fate specification. In cooperation with TP53 potentiates transcriptional activation of BAX promoter activity increasing neuronal cell apoptosis. Negatively regulates BAX promoter activity in the absence of TP53. Acts as a transcriptional coactivator via its interaction with the transcription factor ESR1 by enhancing its effect on estrogen response element (ERE)-containing promoter. Antagonizes the transcriptional stimulatory activity of POU4F1 by preventing its binding to an octamer motif. Involved in TNFSF11-mediated terminal osteoclast differentiation. |
| Protein Name | Pou Domain - Class 4 - Transcription Factor 2Brain-Specific Homeobox/Pou Domain Protein 3bBrain-3bBrn-3b |
| Database Links | Reactome: R-HSA-6804759 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasm |
| Alternative Antibody Names | Anti-Pou Domain - Class 4 - Transcription Factor 2 antibodyAnti-Brain-Specific Homeobox/Pou Domain Protein 3b antibodyAnti-Brain-3b antibodyAnti-Brn-3b antibodyAnti-POU4F2 antibodyAnti-BRN3B antibody |
Information sourced from Uniprot.org