• STJ72235 (1µg/ml) staining of Mouse Spinal Cord (A) and Brain (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72235 Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72235 Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-POU3F3/BRN1/OCT8 antibody (Internal) (STJ72235)

SKU:
STJ72235-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-POU3F3/BRN1/OCT8 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IF-Strong expression of the protein seen in the nuclei of Neuro-2a cells. 10µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: POU3F3
Gene ID: 5455
Uniprot ID: PO3F3_HUMAN
Immunogen Region: Internal
Accession Number: NP_006227.1
Immunogen Sequence: HMLSHAHQWVTAL
Function Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction.the enhancer element contains the octamer motif (5'-ATTTGCAT-3').
Protein Name Pou Domain - Class 3 - Transcription Factor 3
Brain-Specific Homeobox/Pou Domain Protein 1
Brain-1
Brn-1
Octamer-Binding Protein 8
Oct-8
Octamer-Binding Transcription Factor 8
Otf-8
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 3 - Transcription Factor 3 antibody
Anti-Brain-Specific Homeobox/Pou Domain Protein 1 antibody
Anti-Brain-1 antibody
Anti-Brn-1 antibody
Anti-Octamer-Binding Protein 8 antibody
Anti-Oct-8 antibody
Anti-Octamer-Binding Transcription Factor 8 antibody
Anti-Otf-8 antibody
Anti-POU3F3 antibody
Anti-BRN1 antibody
Anti-OTF8 antibody

Information sourced from Uniprot.org

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