Western blot analysis of various lysates, using POU2F3 Rabbit polyclonal antibody (STJ112094) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POU2F3 antibody (1-180) (STJ112094)

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STJ112094
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2F3
Gene ID: 25833
Uniprot ID: PO2F3_HUMAN
Immunogen Region: 1-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human POU2F3 (NP_055167.2).
Immunogen Sequence: MVNLESMHTDIKMSGDVADS TDARSTLSQVEPGNDRNGLD FNRQIKTEDLSDSLQQTLSH RPCHLSQGPAMMSGNQMSGL NASPCQDMASLHPLQQLVLV PGHLQSVSQFLLSQTQPGQQ GLQPNLLPFPQQQSGLLLPQ TGPGLASQAFGHPGLPGSSL EPHLEASQHLPVPKHLPSSG
Tissue Specificity Specifically expressed in epidermis and cultured keratinocytes.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and regulates cell type-specific differentiation pathways. Involved in the regulation of keratinocytes differentiation. The POU2F3-POU2AF2/POU2AF3 complex drives the expression of tuft-cell-specific genes, a rare chemosensory cells that coordinate immune and neural functions within mucosal epithelial tissues.
Protein Name Pou Domain - Class 2 - Transcription Factor 3
Octamer-Binding Protein 11
Oct-11
Octamer-Binding Transcription Factor 11
Otf-11
Transcription Factor Pla-1
Transcription Factor Skn-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 2 - Transcription Factor 3 antibody
Anti-Octamer-Binding Protein 11 antibody
Anti-Oct-11 antibody
Anti-Octamer-Binding Transcription Factor 11 antibody
Anti-Otf-11 antibody
Anti-Transcription Factor Pla-1 antibody
Anti-Transcription Factor Skn-1 antibody
Anti-POU2F3 antibody
Anti-OTF11 antibody
Anti-PLA1 antibody

Information sourced from Uniprot.org

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