Anti-POU2AF1 antibody (150-256 aa) [ABT-BOB1] (STJ196784)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenSynthesized peptide derived from the human BOB.1 at the amino acid range 150-256
General Information
| Short Description | Mouse monoclonal anti-POU domain class 2-associating factor 1 (150-256 aa) for use in IHC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT-BOB1 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:100-500IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POU2AF1 |
| Gene ID | 5450 |
| Uniprot ID | OBF1_HUMAN |
| Immunogen | Synthesized peptide derived from the human BOB.1 at the amino acid range 150-256 |
| Immunogen Region | 150-256 aa |
| Specificity | This antibody detects endogenous levels of human BOB.1. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section |
Additional Info
| Post Translational Modifications | Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. |
| Function | Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator. |
| Protein Name | Pou Domain Class 2-Associating Factor 1B-Cell-Specific Coactivator Obf-1Bob-1Oca-BOct-Binding Factor 1 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pou Domain Class 2-Associating Factor 1 antibodyAnti-B-Cell-Specific Coactivator Obf-1 antibodyAnti-Bob-1 antibodyAnti-Oca-B antibodyAnti-Oct-Binding Factor 1 antibodyAnti-POU2AF1 antibodyAnti-BOB1 antibodyAnti-OBF1 antibody |
Information sourced from Uniprot.org