Anti-PORCN antibody (220-320) (STJ114140)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PORCN (220-320) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PORCN |
| Gene ID | 64840 |
| Uniprot ID | PORCN_HUMAN |
| Immunogen Region | 220-320 |
| Immunogen Sequence | PLNGDRLLRNKKRKARWLRA YESAVSFHFSNYFVGFLSEA TATLAGAGFTEEKDHLEWDL TVSKPLNVELPRSMVEVVTS WNLPMSYWLNNYVFKNALRL G |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_982299.1). |
Additional Info
| Tissue Specificity | Isoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung. |
| Function | Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1(9Z)), to Wnt proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. |
| Protein Name | Protein-Serine O-Palmitoleoyltransferase PorcupineProtein Mg61 |
| Database Links | Reactome: R-HSA-3238698Reactome: R-HSA-5340573 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Protein-Serine O-Palmitoleoyltransferase Porcupine antibodyAnti-Protein Mg61 antibodyAnti-PORCN antibodyAnti-MG61 antibodyAnti-PORC antibodyAnti-PPN antibody |
Information sourced from Uniprot.org