• Immunofluorescence analysis of U-2 OS cells using CYPOR Rabbit monoclonal antibody (STJ11102230) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CYPOR in paraffin-embedded human colon carcinoma tissue using CYPOR Rabbit monoclonal antibody (STJ11102230) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CYPOR in paraffin-embedded human liver tissue using CYPOR Rabbit monoclonal antibody (STJ11102230) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CYPOR in paraffin-embedded mouse intestin tissue using CYPOR Rabbit monoclonal antibody (STJ11102230) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CYPOR in paraffin-embedded Human lung adenocarcinoma tissue using CYPOR Rabbit monoclonal antibody (STJ11102230) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CYPOR in paraffin-embedded mouse kidney tissue using CYPOR Rabbit monoclonal antibody (STJ11102230) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using CYPOR Rabbit monoclonal antibody (STJ11102230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-POR antibody (100-200) [S0MR] (STJ11102230)

SKU:
STJ11102230

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CYPOR (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POR
Gene ID: 5447
Uniprot ID: NCPR_HUMAN
Immunogen Region: 100-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human CYPOR (P16435).
Immunogen Sequence: KDAHRYGMRGMSADPEEYDL ADLSSLPEIDNALVVFCMAT YGEGDPTDNAQDFYDWLQET DVDLSGVKFAVFGLGNKTYE HFNAMGKYVDKRLEQLGAQR I
Function This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Protein Name Nadph--Cytochrome P450 Reductase
Cpr
P450r
Database Links Reactome: R-HSA-211897
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Nadph--Cytochrome P450 Reductase antibody
Anti-Cpr antibody
Anti-P450r antibody
Anti-POR antibody
Anti-CYPOR antibody

Information sourced from Uniprot.org

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