• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g POLR2D antibody (STJ25047). Western blot was performed from the immunoprecipitate using POLR2D antibody (STJ25047) at a dilution of 1:1000.
  • Immunofluorescence analysis of A549 cells using POLR2D Rabbit polyclonal antibody (STJ25047). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Western blot analysis of various lysates using POLR2D Rabbit polyclonal antibody (STJ25047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-POLR2D antibody (1-142) (STJ25047)

SKU:
STJ25047

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLR2D (1-142) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR2D
Gene ID: 5433
Uniprot ID: RPB4_HUMAN
Immunogen Region: 1-142
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
Immunogen Sequence: MAAGGSDPRAGDVEEDASQL IFPKEFETAETLLNSEVHML LEHRKQQNESAEDEQELSEV FMKTLNYTARFSRFKNRETI ASVRSLLLQKKLHKFELACL ANLCPETAEESKALIPSLEG RFEDEELQQILDDIQTKRSF QY
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RPB4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
Protein Name Dna-Directed Rna Polymerase Ii Subunit Rpb4
Rna Polymerase Ii Subunit B4
Dna-Directed Rna Polymerase Ii Subunit D
Rna Polymerase Ii 16 Kda Subunit
Rpb16
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-168325
Reactome: R-HSA-203927
Reactome: R-HSA-5578749
Reactome: R-HSA-5601884
Reactome: R-HSA-5617472
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Reactome: R-HSA-8851708
Reactome: R-HSA-9018519
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb4 antibody
Anti-Rna Polymerase Ii Subunit B4 antibody
Anti-Dna-Directed Rna Polymerase Ii Subunit D antibody
Anti-Rna Polymerase Ii 16 Kda Subunit antibody
Anti-Rpb16 antibody
Anti-POLR2D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance