Anti-POLQ antibody (181-230 aa) (STJ92741)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human POLQ at the amino acid range 181-230
General Information
| Short Description | Rabbit polyclonal anti-DNA polymerase theta (181-230 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLQ |
| Gene ID | 10721 |
| Uniprot ID | DPOLQ_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human POLQ at the amino acid range 181-230 |
| Immunogen Region | 181-230 aa |
| Specificity | DNA pol Theta Polyclonal Antibody detects endogenous levels of DNA pol Theta protein. |
Additional Info
| Post Translational Modifications | Phosphorylated by PLK1.promoting interaction with TOPBP1 and recruitment to DNA damage sites. |
| Function | Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. The polymerase lacks proofreading activity and is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates. When the ends of a break do not contain terminal microhomology must identify embedded complementary sequences through a scanning step. Also acts as a DNA helicase, promoting dissociation of the replication protein A complex (RPA/RP-A), composed of RPA1, RPA2 and RPA3, from resected double-strand breaks to allow their annealing and subsequent joining by MMEJ. Removal of RPA/RP-A complex proteins prevents RAD51 accumulation at resected ends, thereby inhibiting homology-recombination repair (HR) pathway. Also shows RNA-directed DNA polymerase activity to mediate DNA repair in vitro.however this activity needs additional evidence in vivo. May also have lyase activity. Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. POLQ-mediated end joining activity is involved in random integration of exogenous DNA hampers. |
| Protein Name | Dna Polymerase ThetaDna Polymerase Eta Includes - Helicase Polq - Dna Polymerase PolqRna-Directed Dna Polymerase Polq |
| Database Links | Reactome: R-HSA-5685939 |
| Cellular Localisation | NucleusChromosomeEnriched In Chromatin In Response To Ultaviolet (Uv) LightBinds To Chromatin During Early G1Recruited To Dna Damage SitesSuch As Double-Stranded Breaks (Dsbs)Following Interaction With Topbp1 And Rhno1 |
| Alternative Antibody Names | Anti-Dna Polymerase Theta antibodyAnti-Dna Polymerase Eta Includes - Helicase Polq - Dna Polymerase Polq antibodyAnti-Rna-Directed Dna Polymerase Polq antibodyAnti-POLQ antibodyAnti-POLH antibody |
Information sourced from Uniprot.org