• Western blot analysis of extracts of various cell lines, using POLL antibody (STJ110706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Western blot analysis of extracts of various cell lines, using POLL antibody (STJ110706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-POLL antibody (421-575) (STJ110706)

SKU:
STJ110706

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLL (421-575) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLL
Gene ID: 27343
Uniprot ID: DPOLL_HUMAN
Immunogen Region: 421-575
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 421-575 of human POLL (NP_037406.1).
Immunogen Sequence: GKATCGDVDVLITHPDGRSH RGIFSRLLDSLRQEGFLTDD LVSQEENGQQQKYLGVCRLP GPGRRHRRLDIIVVPYSEFA CALLYFTGSAHFNRSMRALA KTKGMSLSEHALSTAVVRNT HGCKVGPGRVLPTPTEKDVF RLLGLPYREPAERDW
Tissue Specificity Expressed in a number of tissues. Abundant in testis.
Function DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Protein Name Dna Polymerase Lambda
Pol Lambda
Dna Polymerase Beta-2
Pol Beta2
Dna Polymerase Kappa
Database Links Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Chromosome
Accumulates At Sites Of Dna Damage
Alternative Antibody Names Anti-Dna Polymerase Lambda antibody
Anti-Pol Lambda antibody
Anti-Dna Polymerase Beta-2 antibody
Anti-Pol Beta2 antibody
Anti-Dna Polymerase Kappa antibody
Anti-POLL antibody

Information sourced from Uniprot.org

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