• Western blot analysis of extracts of various cell lines, using POLE4 antibody (STJ111924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using POLE4 antibody (STJ111924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLE4 antibody (1-117) (STJ111924)

SKU:
STJ111924

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLE4 (1-117) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLE4
Gene ID: 56655
Uniprot ID: DPOE4_HUMAN
Immunogen Region: 1-117
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLE4 (NP_063949.2).
Immunogen Sequence: MAAAAAAGSGTPREEEGPAG EAAASQPQAPTSVPGARLSR LPLARVKALVKADPDVTLAG QEAIFILARAAELFVETIAK DAYCCAQQGKRKTLQRRDLD NAIEAVDEFAFLEGTLD
Function Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication.
Protein Name Dna Polymerase Epsilon Subunit 4
Dna Polymerase Ii Subunit 4
Dna Polymerase Epsilon Subunit P12
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-5651801
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-68952
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Polymerase Epsilon Subunit 4 antibody
Anti-Dna Polymerase Ii Subunit 4 antibody
Anti-Dna Polymerase Epsilon Subunit P12 antibody
Anti-POLE4 antibody

Information sourced from Uniprot.org

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