• Western blot analysis of lysates from NIH/3T3 cells, using DNA Polymerase beta Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of K562 cells using DNA pol Beta Polyclonal Antibody
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DNA Polymerase beta Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using DNA pol Beta Polyclonal Antibody

Anti-POLB antibody (286-335 aa) (STJ92734)

SKU:
STJ92734

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA polymerase beta (286-335 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLB
Gene ID: 5423
Uniprot ID: DPOLB_HUMAN
Immunogen Region: 286-335 aa
Specificity: DNA pol Beta Polyclonal Antibody detects endogenous levels of DNA pol Beta protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DNA Polymerase beta at the amino acid range 286-335
Function Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase).through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.
Protein Name Dna Polymerase Beta
5'-Deoxyribose-Phosphate Lyase
5'-Drp Lyase
Ap Lyase
Database Links Reactome: R-HSA-110362
Reactome: R-HSA-110373
Reactome: R-HSA-110381
Reactome: R-HSA-5649702
Reactome: R-HSA-5651801
Reactome: R-HSA-5689880
Reactome: R-HSA-73930
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic In Normal Conditions
Translocates To The Nucleus Following Dna Damage
Alternative Antibody Names Anti-Dna Polymerase Beta antibody
Anti-5'-Deoxyribose-Phosphate Lyase antibody
Anti-5'-Drp Lyase antibody
Anti-Ap Lyase antibody
Anti-POLB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance