• STJ71107 (0. 2µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PNPLA2/ATGL antibody (Internal) (STJ71107)

SKU:
STJ71107-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PNPLA2/ATGL (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.2-0.6µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PNPLA2
Gene ID: 57104
Uniprot ID: PLPL2_HUMAN
Immunogen Region: Internal
Accession Number: NP_065109.1
Immunogen Sequence: PARPHGPEDKDQ
Post Translational Modifications Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity. Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation. Ubiquitinated by PEX2 in response to reactive oxygen species (ROS), leading to its degradation.
Function Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Also possesses acylglycerol transacylase and phospholipase A2 activities. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes.
Protein Name Patatin-Like Phospholipase Domain-Containing Protein 2
Adipose Triglyceride Lipase
Calcium-Independent Phospholipase A2-Zeta
Ipla2-Zeta
Desnutrin
Pigment Epithelium-Derived Factor Receptor
Pedf-R
Tts2.2
Transport-Secretion Protein 2
Tts2
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Lipid Droplet
Cell Membrane
Multi-Pass Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Patatin-Like Phospholipase Domain-Containing Protein 2 antibody
Anti-Adipose Triglyceride Lipase antibody
Anti-Calcium-Independent Phospholipase A2-Zeta antibody
Anti-Ipla2-Zeta antibody
Anti-Desnutrin antibody
Anti-Pigment Epithelium-Derived Factor Receptor antibody
Anti-Pedf-R antibody
Anti-Tts2.2 antibody
Anti-Transport-Secretion Protein 2 antibody
Anti-Tts2 antibody
Anti-PNPLA2 antibody
Anti-ATGL antibody
Anti-FP17548 antibody

Information sourced from Uniprot.org

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