• Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (STJ28776). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using PNKP antibody (STJ28776) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using PNKP antibody (STJ28776) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using PNKP antibody (STJ28776) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PNKP antibody (STJ28776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PNKP antibody (60-320) (STJ28776)

SKU:
STJ28776

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNKP (60-320) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNKP
Gene ID: 11284
Uniprot ID: PNKP_HUMAN
Immunogen Region: 60-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
Immunogen Sequence: RTVAVKQLGVNPSTTGTQEL KPGLEGSLGVGDTLYLVNGL HPLTLRWEETRTPESQPDTP PGTPLVSQDEKRDAELPKKR MRKSNPGWENLEKLLVFTAA GVKPQGKVAGFDLDGTLITT RSGKVFPTGPSDWRILYPEI PRKLRELEAEGYKLVIFTNQ MSIGRGKLPAEEFKAKVEAV VEKLGVPFQVLVATHAGLYR KPVTGMWDHLQEQANDGTPI SIGDSIFVGDAAGRPANWA
Tissue Specificity Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver.
Function Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.
Protein Name Bifunctional Polynucleotide Phosphatase/Kinase
Dna 5'-Kinase/3'-Phosphatase
Polynucleotide Kinase-3'-Phosphatase Includes - Polynucleotide 3'-Phosphatase
2'(3'-Polynucleotidase - Polynucleotide 5'-Hydroxyl-Kinase
Database Links Reactome: R-HSA-5649702
Cellular Localisation Nucleus
Chromosome
Localizes To Site Of Double-Strand Breaks
Alternative Antibody Names Anti-Bifunctional Polynucleotide Phosphatase/Kinase antibody
Anti-Dna 5'-Kinase/3'-Phosphatase antibody
Anti-Polynucleotide Kinase-3'-Phosphatase Includes - Polynucleotide 3'-Phosphatase antibody
Anti-2'(3'-Polynucleotidase - Polynucleotide 5'-Hydroxyl-Kinase antibody
Anti-PNKP antibody

Information sourced from Uniprot.org

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