| Short Description : | Rabbit polyclonal PNKP (60-320) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PNKP |
| Gene ID: | 11284 |
| Uniprot ID: | PNKP_HUMAN |
| Immunogen Region: | 60-320 |
| Immunogen Sequence: | RTVAVKQLGVNPSTTGTQEL KPGLEGSLGVGDTLYLVNGL HPLTLRWEETRTPESQPDTP PGTPLVSQDEKRDAELPKKR MRKSNPGWENLEKLLVFTAA GVKPQGKVAGFDLDGTLITT RSGKVFPTGPSDWRILYPEI PRKLRELEAEGYKLVIFTNQ MSIGRGKLPAEEFKAKVEAV VEKLGVPFQVLVATHAGLYR KPVTGMWDHLQEQANDGTPI SIGDSIFVGDAAGRPANWA |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2). |
| Tissue Specificity | Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver. |
| Function | Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. |
| Protein Name | Bifunctional Polynucleotide Phosphatase/KinaseDna 5'-Kinase/3'-PhosphatasePolynucleotide Kinase-3'-Phosphatase Includes - Polynucleotide 3'-Phosphatase2'(3'-Polynucleotidase - Polynucleotide 5'-Hydroxyl-Kinase |
| Database Links | Reactome: R-HSA-5649702 |
| Cellular Localisation | NucleusChromosomeLocalizes To Site Of Double-Strand Breaks |
| Alternative Antibody Names | Anti-Bifunctional Polynucleotide Phosphatase/Kinase antibodyAnti-Dna 5'-Kinase/3'-Phosphatase antibodyAnti-Polynucleotide Kinase-3'-Phosphatase Includes - Polynucleotide 3'-Phosphatase antibodyAnti-2'(3'-Polynucleotidase - Polynucleotide 5'-Hydroxyl-Kinase antibodyAnti-PNKP antibody |
Information sourced from Uniprot.org
