Anti-PMS2 antibody (220-270) {FITC} (STJ502564)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationFITC
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 220-270 on human Mismatch Repair endonuclease PMS2 protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-PMS2 (220-270) is suitable for use in ELISA and Western Blot research applications. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | FITC |
| Concentration | 0.65-0.69 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500-1:1, 000DB: 1:20, 000-1:50, 000ELISA: 1:20, 000-1:50, 000IP: 1:50-1:200IHC: 1:50-1:200 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PMS2 |
| Gene ID | 5395 |
| Uniprot ID | PMS2_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 220-270 on human Mismatch Repair endonuclease PMS2 protein. |
| Immunogen Region | 220-270 |
Additional Info
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair. |
| Protein Name | Mismatch Repair Endonuclease Pms2Dna Mismatch Repair Protein Pms2Pms1 Protein Homolog 2 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5545483Reactome: R-HSA-5632987Reactome: R-HSA-6796648 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mismatch Repair Endonuclease Pms2 antibodyAnti-Dna Mismatch Repair Protein Pms2 antibodyAnti-Pms1 Protein Homolog 2 antibodyAnti-PMS2 antibodyAnti-PMSL2 antibody |
Information sourced from Uniprot.org