• Immunofluorescence analysis of L929 cells using PMM2 antibody (STJ25035) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PMM2 antibody (STJ25035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PMM2 antibody (1-246) (STJ25035)

SKU:
STJ25035

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PMM2 (1-246) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PMM2
Gene ID: 5373
Uniprot ID: PMM2_HUMAN
Immunogen Region: 1-246
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PMM2 (NP_000294.1).
Immunogen Sequence: MAAPGPALCLFDVDGTLTAP RQKITKEMDDFLQKLRQKIK IGVVGGSDFEKVQEQLGNDV VEKYDYVFPENGLVAYKDGK LLCRQNIQSHLGEALIQDLI NYCLSYIAKIKLPKKRGTFI EFRNGMLNVSPIGRSCSQEE RIEFYELDKKENIRQKFVAD LRKEFAGKGLTFSIGGQISF DVFPDGWDKRYCLRHVENDG YKTIYFFGDKTMPGGNDHEI FTDPRTMGYSVTAPEDTRR
Function Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Protein Name Phosphomannomutase 2
Pmm 2
Database Links Reactome: R-HSA-4043911
Reactome: R-HSA-446205
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Phosphomannomutase 2 antibody
Anti-Pmm 2 antibody
Anti-PMM2 antibody

Information sourced from Uniprot.org

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