| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Protein PML (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PML |
| Gene ID: |
5371 |
| Uniprot ID: |
PML_HUMAN |
| Immunogen Region: |
11-60 aa |
| Specificity: |
PML Polyclonal Antibody detects endogenous levels of PML protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human PML at the amino acid range 11-60 |
| Function | Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Inhibits EIF4E-mediated mRNA nuclear export by reducing EIF4E affinity for the 5' 7-methylguanosine (m7G) cap of target mRNAs. Isoform PML-4 has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Isoform PML-4 also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, regulates double-strand break repair in gamma-irradiation-induced DNA damage responses via its interaction with WRN, acts as a negative regulator of telomerase by interacting with TERT, and regulates PER2 nuclear localization and circadian function. Isoform PML-6 inhibits specifically the activity of the tetrameric form of PKM. The nuclear isoforms (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5) in concert with SATB1 are involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Isoform PML-2 is required for efficient IFN-gamma induced MHC II gene transcription via regulation of CIITA. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. PML also regulates transcription activity of ELF4 and can act as an important mediator for TNF-alpha- and IFN-alpha-mediated inhibition of endothelial cell network formation and migration. Exhibits antiviral activity against both DNA and RNA viruses. The antiviral activity can involve one or several isoform(s) and can be enhanced by the permanent PML-NB-associated protein DAXX or by the recruitment of p53/TP53 within these structures. Isoform PML-4 restricts varicella zoster virus (VZV) via sequestration of virion capsids in PML-NBs thereby preventing their nuclear egress and inhibiting formation of infectious virus particles. The sumoylated isoform PML-4 restricts rabies virus by inhibiting viral mRNA and protein synthesis. The cytoplasmic isoform PML-14 can restrict herpes simplex virus-1 (HHV-1) replication by sequestering the viral E3 ubiquitin-protein ligase ICP0 in the cytoplasm. Isoform PML-6 shows restriction activity towards human cytomegalovirus (HHV-5) and influenza A virus strains PR8(H1N1) and ST364(H3N2). Sumoylated isoform PML-4 and isoform PML-12 show antiviral activity against encephalomyocarditis virus (EMCV) by promoting nuclear sequestration of viral polymerase (P3D-POL) within PML NBs. Isoform PML-3 exhibits antiviral activity against poliovirus by inducing apoptosis in infected cells through the recruitment and the activation of p53/TP53 in the PML-NBs. Isoform PML-3 represses human foamy virus (HFV) transcription by complexing the HFV transactivator, bel1/tas, preventing its binding to viral DNA. PML may positively regulate infectious hepatitis C viral (HCV) production and isoform PML-2 may enhance adenovirus transcription. Functions as an E3 SUMO-protein ligase that sumoylates (HHV-5) immediate early protein IE1, thereby participating in the antiviral response. Isoforms PML-3 and PML-6 display the highest levels of sumoylation activity. |
| Protein Name | Protein PmlE3 Sumo-Protein Ligase PmlPromyelocytic Leukemia ProteinRing Finger Protein 71Ring-Type E3 Sumo Transferase PmlTripartite Motif-Containing Protein 19Trim19 |
| Database Links | Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-6804758Reactome: R-HSA-877300Reactome: R-HSA-8934593Reactome: R-HSA-8948747Reactome: R-HSA-9609690Reactome: R-HSA-9616222 P29590-4 |
| Cellular Localisation | NucleusNucleoplasmCytoplasmPml BodyNucleolusEndoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideEarly Endosome MembraneIsoform Pml-1 Can Shuttle Between The Nucleus And CytoplasmIsoform Pml-2Isoform Pml-3Isoform Pml-4Isoform Pml-5 And Isoform Pml-6 Are Nuclear Isoforms Whereas Isoform Pml-7 And Isoform Pml-14 Lacking The Nuclear Localization Signal Are Cytoplasmic IsoformsDetected In The Nucleolus After Dna DamageAcetylation At Lys-487 Is Essential For Its Nuclear LocalizationWithin The NucleusMost Of Pml Is Expressed In The Diffuse Nuclear Fraction Of The Nucleoplasm And Only A Small Fraction Is Found In The Matrix-Associated Nuclear Bodies (Pml-Nbs)The Transfer Of Pml From The Nucleoplasm To Pml-Nbs Depends On Its Phosphorylation And SumoylationThe B1 Box And The Ring Finger Are Also Required For The Localization In Pml-NbsAlso Found In Specific Membrane Structures Termed Mitochondria-Associated Membranes (Mams) Which Connect The Endoplasmic Reticulum (Er) And The MitochondriaSequestered In The Cytoplasm By Interaction With Rabies Virus Phosphoprotein |
| Alternative Antibody Names | Anti-Protein Pml antibodyAnti-E3 Sumo-Protein Ligase Pml antibodyAnti-Promyelocytic Leukemia Protein antibodyAnti-Ring Finger Protein 71 antibodyAnti-Ring-Type E3 Sumo Transferase Pml antibodyAnti-Tripartite Motif-Containing Protein 19 antibodyAnti-Trim19 antibodyAnti-PML antibodyAnti-MYL antibodyAnti-PP8675 antibodyAnti-RNF71 antibodyAnti-TRIM19 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
