• Western blot analysis of extracts of Mouse uterus, using PLOD2/LH2 antibody (STJ29026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat uterus, using PLOD2/LH2 antibody (STJ29026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using PLOD2/LH2 antibody (STJ29026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PLOD2 antibody (260-380) (STJ29026)

SKU:
STJ29026

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLOD2 (260-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLOD2
Gene ID: 5352
Uniprot ID: PLOD2_HUMAN
Immunogen Region: 260-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-380 of human PLOD2/LH2 (NP_000926.2).
Immunogen Sequence: ILLNYFGNYVPNSWTQDNGC TLCEFDTVDLSAVDVHPNVS IGVFIEQPTPFLPRFLDILL TLDYPKEALKLFIHNKEVYH EKDIKVFFDKAKHEIKTIKI VGPEENLSQAEARNMGMDFC R
Tissue Specificity Highly expressed in pancreas and muscle. Isoform 1 and isoform 2 are expressed in the majority of the examined cell types. Isoform 2 is specifically expressed in skin, lung, dura and aorta.
Function Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Protein Name Procollagen-Lysine -2-Oxoglutarate 5-Dioxygenase 2
Lysyl Hydroxylase 2
Lh2
Database Links Reactome: R-HSA-1650814
Cellular Localisation Rough Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Lumenal Side
Alternative Antibody Names Anti-Procollagen-Lysine -2-Oxoglutarate 5-Dioxygenase 2 antibody
Anti-Lysyl Hydroxylase 2 antibody
Anti-Lh2 antibody
Anti-PLOD2 antibody

Information sourced from Uniprot.org

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