Anti-PLEKHM1 antibody (STJA0008876)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human PLEKHM1
General Information
| Short Description | Rabbit polyclonal anti-Pleckstrin homology domain-containing family M member 1 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLEKHM1 |
| Gene ID | 9842 |
| Uniprot ID | PKHM1_HUMAN |
| Immunogen | Synthesized peptide derived from human PLEKHM1 |
| Specificity | This antibody detects endogenous levels of PLEKHM1 at Human, Mouse, Rat |
Additional Info
| Function | Acts as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Acts as a dual effector of RAB7A and ARL8B that simultaneously binds these GTPases, bringing about clustering and fusion of late endosomes and lysosomes. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Interaction with Arl8b is a crucial factor in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts. May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7. May have a role in sialyl-lex-mediated transduction of apoptotic signals. Involved in bone resorption. (Microbial infection) In case of infection contributes to Salmonella typhimurium pathogenesis by supporting the integrity of the Salmonella-containing vacuole (SCV) probably in concert with the HOPS complex and Rab7. |
| Protein Name | Pleckstrin Homology Domain-Containing Family M Member 1Ph Domain-Containing Family M Member 1162 Kda Adapter ProteinAp162 |
| Database Links | |
| Cellular Localisation | Autolysosome MembraneEndosome MembraneLate Endosome MembraneLysosome MembraneIn Case Of Infection Colocalizes With Salmonella Typhimurium Sifa In Proximity Of Salmonella-Containing Vacuole (Scv) |
| Alternative Antibody Names | Anti-Pleckstrin Homology Domain-Containing Family M Member 1 antibodyAnti-Ph Domain-Containing Family M Member 1 antibodyAnti-162 Kda Adapter Protein antibodyAnti-Ap162 antibodyAnti-PLEKHM1 antibodyAnti-KIAA0356 antibody |
Information sourced from Uniprot.org