| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mitochondrial cardiolipin hydrolase (29-79 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLD6 |
| Gene ID: |
201164 |
| Uniprot ID: |
PLD6_HUMAN |
| Immunogen Region: |
29-79 aa |
| Specificity: |
This antibody detects endogenous levels of PLD6 at Human/Mouse |
| Immunogen: |
Synthesized peptide derived from the human PLD6 at the amino acid range 29-79 |
| Function | Presents phospholipase and nuclease activities, depending on the different physiological conditions. Interaction with Mitoguardin (MIGA1 or MIGA2) affects the dimer conformation, facilitating the lipase activity over the nuclease activity. Plays a key role in mitochondrial fusion and fission via its phospholipase activity. In its phospholipase role, it uses the mitochondrial lipid cardiolipin as substrate to generate phosphatidate (PA or 1,2-diacyl-sn-glycero-3-phosphate), a second messenger signaling lipid. Production of PA facilitates Mitofusin-mediated fusion, whereas the cleavage of PA by the Lipin family of phosphatases produces diacylgycerol (DAG) which promotes mitochondrial fission. Both Lipin and DAG regulate mitochondrial dynamics and membrane fusion/fission, important processes for adapting mitochondrial metabolism to changes in cell physiology. Mitochondrial fusion enables cells to cope with the increased nucleotide demand during DNA synthesis. Mitochondrial function and dynamics are closely associated with biological processes such as cell growth, proliferation, and differentiation. Mediator of MYC activity, promotes mitochondrial fusion and activates AMPK which in turn inhibits YAP/TAZ, thereby inducing cell growth and proliferation. The endonuclease activity plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. Implicated in spermatogenesis and sperm fertility in testicular germ cells, its single strand-specific nuclease activity is critical for the biogenesis/maturation of PIWI-interacting RNA (piRNA). MOV10L1 selectively binds to piRNA precursors and funnels them to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins. Cleaves either DNA or RNA substrates with similar affinity, producing a 5' phosphate end, in this way it participates in the processing of primary piRNA transcripts. piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. PA may act as signaling molecule in the recognition/transport of the precursor RNAs of primary piRNAs. Interacts with tesmin in testes, suggesting a role in spermatogenesis via association with its interacting partner. |
| Protein Name | Mitochondrial Cardiolipin HydrolaseCholine Phosphatase 6Mitochondrial PhospholipaseMitopldPhosphatidylcholine-Hydrolyzing Phospholipase D6Phospholipase D6Pld6Protein Zucchini Homolog |
| Database Links | Reactome: R-HSA-1483148Reactome: R-HSA-1483166Reactome: R-HSA-5601884 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane ProteinGolgi ApparatusLocalization In The Mitochondrial Outer Membrane Is Found In Different Cell Types Where Phospholipase Is The Predominant ActivityHoweverIn Pachytene Spermatocytes And Spermatids Of Mouse Testes Where Nuclease Activity Is PredominantLocalization Is Restricted To The GolgiSuggesting This Enzyme Is Localized In Different Subcellular Compartments Depending On The Role (Phospholipase Or Nuclease) It Needs To Play In Each Cell Type And Developmental Stage |
| Alternative Antibody Names | Anti-Mitochondrial Cardiolipin Hydrolase antibodyAnti-Choline Phosphatase 6 antibodyAnti-Mitochondrial Phospholipase antibodyAnti-Mitopld antibodyAnti-Phosphatidylcholine-Hydrolyzing Phospholipase D6 antibodyAnti-Phospholipase D6 antibodyAnti-Pld6 antibodyAnti-Protein Zucchini Homolog antibodyAnti-PLD6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
