| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PLCD4 (1-290) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLCD4 |
| Gene ID: |
84812 |
| Uniprot ID: |
PLCD4_HUMAN |
| Immunogen Region: |
1-290 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PLCD4 (NP_116115.1). |
| Immunogen Sequence: |
MASLLQDQLTTDQDLLLMQE GMPMRKVRSKSWKKLRYFRL QNDGMTVWHARQARGSAKPS FSISDVETIRNGHDSELLRS LAEELPLEQGFTIVFHGRRS NLDLMANSVEEAQIWMRGLQ LLVDLVTSMDHQERLDQWLS DWFQRGDKNQDGKMSFQEVQ RLLHLMNVEMDQEYAFSLFQ AADTSQSGTLEGEEFVQFYK ALTKRAEVQELFESFSADGQ KLTLLEFLDFLQEEQKERD |
| Tissue Specificity | Highly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells. |
| Function | Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. Isoform 2: Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3. |
| Protein Name | 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Delta-4Hplcd4Phosphoinositide Phospholipase C-Delta-4Phospholipase C-Delta-4Plc-Delta-4 |
| Database Links | Reactome: R-HSA-1855204 |
| Cellular Localisation | MembranePeripheral Membrane ProteinNucleusCytoplasmEndoplasmic ReticulumLocalizes Primarily To Intracellular Membranes Mostly To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Delta-4 antibodyAnti-Hplcd4 antibodyAnti-Phosphoinositide Phospholipase C-Delta-4 antibodyAnti-Phospholipase C-Delta-4 antibodyAnti-Plc-Delta-4 antibodyAnti-PLCD4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MSH6 knockout (KO) HeLa cells, using [KO Validated] MSH6 Rabbit polyclonal antibody (STJ119980) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98765/382817/STJ119980_1__09948.1713151312.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MSH6 knockout (KO) HeLa cells, using [KO Validated] MSH6 Rabbit polyclonal antibody (STJ119980) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")