• Biotinylated STJ70200 (2µg/ml) staining of A431 lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Plakoglobin/Gamma-catenin, Biotinylated antibody (C-Term) {Biotin} (STJ73259)

SKU:
STJ73259-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Plakoglobin/Gamma-catenin, Biotinylated (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: JUP
Gene ID: 3728
Uniprot ID: PLAK_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002221.1, NP_068831.1
Specificity: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
Immunogen Sequence: GLRPPYPTADHMLA.
Post Translational Modifications May be phosphorylated by FER.
Function Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Protein Name Junction Plakoglobin
Catenin Gamma
Desmoplakin Iii
Desmoplakin-3
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-5218920
Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013409
Reactome: R-HSA-9762292
Reactome: R-HSA-9764302
Reactome: R-HSA-9833576
Cellular Localisation Cell Junction
Adherens Junction
Desmosome
Cytoplasm
Cytoskeleton
Membrane
Peripheral Membrane Protein
Cytoplasmic In A Soluble And Membrane-Associated Form
Alternative Antibody Names Anti-Junction Plakoglobin antibody
Anti-Catenin Gamma antibody
Anti-Desmoplakin Iii antibody
Anti-Desmoplakin-3 antibody
Anti-JUP antibody
Anti-CTNNG antibody
Anti-DP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance