| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Plakoglobin/Gamma-catenin (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 80kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | JUP |
| Gene ID: | 3728 |
| Uniprot ID: | PLAK_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_002221.1, NP_068831.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1. |
| Immunogen Sequence: | GLRPPYPTADHMLA |
| Post Translational Modifications | May be phosphorylated by FER. |
| Function | Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. |
| Protein Name | Junction Plakoglobin Catenin Gamma Desmoplakin Iii Desmoplakin-3 |
| Database Links | Reactome: R-HSA-418990 Reactome: R-HSA-5218920 Reactome: R-HSA-6798695 Reactome: R-HSA-6805567 Reactome: R-HSA-6809371 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013406 Reactome: R-HSA-9013407 Reactome: R-HSA-9013409 Reactome: R-HSA-9762292 Reactome: R-HSA-9764302 Reactome: R-HSA-9833576 |
| Cellular Localisation | Cell Junction Adherens Junction Desmosome Cytoplasm Cytoskeleton Cell Membrane Peripheral Membrane Protein Nucleus Cytoplasmic In A Soluble And Membrane-Associated Form Colocalizes With Dsg4 At Desmosomes |
| Alternative Antibody Names | Anti-Junction Plakoglobin antibody Anti-Catenin Gamma antibody Anti-Desmoplakin Iii antibody Anti-Desmoplakin-3 antibody Anti-JUP antibody Anti-CTNNG antibody Anti-DP3 antibody |
Information sourced from Uniprot.org