Anti-PLA2G7 antibody (53-64) (STJ72956)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PLA2G7 (53-64) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 100kDa band in Human Liver lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PLA2G7 |
| Gene ID | 7941 |
| Uniprot ID | PAFA_HUMAN |
| Accession Number | NP_005075.3 |
| Immunogen Region | 53-64 |
| Immunogen Sequence | QTKIPRGNGPYS |
| Specificity | Reported variants represent identical protein: NP_005075.3, NP_001161829.1 |
Additional Info
| Post Translational Modifications | N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles. |
| Function | Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage. |
| Protein Name | Platelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase1-Alkyl-2-Acetylglycerophosphocholine Esterase2-Acetyl-1-Alkylglycerophosphocholine EsteraseGroup-Viia Phospholipase A2Gviia-Pla2Ldl-Associated Phospholipase A2Ldl-Pla(2Paf 2-Acylhydrolase |
| Database Links | Reactome: R-HSA-422085 |
| Cellular Localisation | SecretedExtracellular SpaceAssociates With Both Ldl And Hdl Particles In PlasmaMainly Associates With Pro-Inflammatory Electronegative Ldl Particles |
| Alternative Antibody Names | Anti-Platelet-Activating Factor Acetylhydrolase antibodyAnti-Paf Acetylhydrolase antibodyAnti-1-Alkyl-2-Acetylglycerophosphocholine Esterase antibodyAnti-2-Acetyl-1-Alkylglycerophosphocholine Esterase antibodyAnti-Group-Viia Phospholipase A2 antibodyAnti-Gviia-Pla2 antibodyAnti-Ldl-Associated Phospholipase A2 antibodyAnti-Ldl-Pla(2 antibodyAnti-Paf 2-Acylhydrolase antibodyAnti-PLA2G7 antibodyAnti-PAFAH antibody |
Information sourced from Uniprot.org