• Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 30', using c-PLA2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using c-PLA2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, cPLA2 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PLA2G4A antibody (471-520 aa) (STJ92449)

SKU:
STJ92449

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytosolic phospholipase A2 (471-520 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLA2G4A
Gene ID: 5321
Uniprot ID: PA24A_HUMAN
Immunogen Region: 471-520 aa
Specificity: cPLA2 Polyclonal Antibody detects endogenous levels of cPLA2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human c-PLA2 at the amino acid range 471-520
Post Translational Modifications Phosphorylated at both Ser-505 and Ser-727 in response to mitogenic stimuli.
Function Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production. Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific. Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis.
Protein Name Cytosolic Phospholipase A2
Cpla2
Phospholipase A2 Group Iva Includes - Phospholipase A2
Phosphatidylcholine 2-Acylhydrolase - Lysophospholipase
Database Links Reactome: R-HSA-111995
Reactome: R-HSA-1482788
Reactome: R-HSA-1482798
Reactome: R-HSA-1482801
Reactome: R-HSA-1482839
Reactome: R-HSA-1482922
Reactome: R-HSA-1482925
Reactome: R-HSA-1483115
Reactome: R-HSA-1483166
Reactome: R-HSA-2142753
Reactome: R-HSA-418592
Reactome: R-HSA-432142
Reactome: R-HSA-6811436
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Nucleus Envelope
Translocates To Intracellular Membranes In A Calcium-Dependent Way
Alternative Antibody Names Anti-Cytosolic Phospholipase A2 antibody
Anti-Cpla2 antibody
Anti-Phospholipase A2 Group Iva Includes - Phospholipase A2 antibody
Anti-Phosphatidylcholine 2-Acylhydrolase - Lysophospholipase antibody
Anti-PLA2G4A antibody
Anti-CPLA2 antibody
Anti-PLA2G4 antibody

Information sourced from Uniprot.org

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