Anti-PLA2G2A antibody [R05-5J7] (STJA0037414)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human PLA2G2A
General Information
| Short Description | Rabbit monoclonal anti-PLA2G2A for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-5J7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PLA2G2A |
| Gene ID | 5320 |
| Uniprot ID | PA2GA_HUMAN |
| Immunogen | A synthesized peptide derived from human PLA2G2A |
Additional Info
| Tissue Specificity | Expressed in various tissues including heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, prostate, ovary, colon and small intestine. Not detected in lymphoid organs and brain. Expressed in platelets (at protein level). |
| Function | Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes. Simultaneously, by compromising mitochondrial membrane integrity, promotes the release in circulation of potent damage-associated molecular pattern molecules that activate the innate immune response. Plays a stem cell regulator role in the intestinal crypt. Within intracellular compartment mediates Paneth cell differentiation and its stem cell supporting functions by inhibiting Wnt signaling pathway in intestinal stem cell (ICS). Secreted in the intestinal lumen upon inflammation, acts in an autocrine way and promotes prostaglandin E2 synthesis that stimulates Wnt signaling pathway in ICS cells and tissue regeneration. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner. |
| Protein Name | Phospholipase A2 - Membrane AssociatedGiic Spla2Group Iia Phospholipase A2Non-Pancreatic Secretory Phospholipase A2Nps-Pla2Phosphatidylcholine 2-Acylhydrolase 2a |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482801Reactome: R-HSA-1482839Reactome: R-HSA-1482922Reactome: R-HSA-1482925Reactome: R-HSA-1483166Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedCell MembranePeripheral Membrane ProteinMitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Phospholipase A2 - Membrane Associated antibodyAnti-Giic Spla2 antibodyAnti-Group Iia Phospholipase A2 antibodyAnti-Non-Pancreatic Secretory Phospholipase A2 antibodyAnti-Nps-Pla2 antibodyAnti-Phosphatidylcholine 2-Acylhydrolase 2a antibodyAnti-PLA2G2A antibodyAnti-PLA2B antibodyAnti-PLA2L antibodyAnti-RASF-A antibody |
Information sourced from Uniprot.org