• Western blot analysis of various lysates, using PKM2-specific antibody (STJ11104833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PKM antibody (380-480) [S4833RM] (STJ11104833)

SKU:
STJ11104833

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Pyruvate Kinase Pkm (380-480) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4833RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PKM
Gene ID: 5315
Uniprot ID: KPYM_HUMAN
Immunogen Region: 380-480
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 380-480 of human PKM2-specific (NP_002645.3).
Immunogen Sequence: LIAREAEAAIYHLQLFEELR RLAPITSDPTEATAVGAVEA SFKCCSGAIIVLTKSGRSAH QVARYRPRAPIIAVTRNPQT ARQAHLYRGIFPVLCKDPVQ E
Tissue Specificity Isoform M2: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Isoform M1: Expressed in adult tissues. Not expressed in tumor cells.
Post Translational Modifications ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Isoform M2: Acetylated at Lys-433 by EP300, leading to impair phosphoenolpyruvate substrate-binding and promote its homodimerization and subsequent translocation to the nucleus. Deacetylation at Lys-433 by SIRT6 promotes its nuclear export into the cytoplasm, leading to suppress its nuclear localization and oncogenic function. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
Function Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Isoform M2: Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity. In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase. Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase. Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription. Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis. Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages. May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a role in caspase independent cell death of tumor cells. Isoform M1: Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth. In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity.
Protein Name Pyruvate Kinase Pkm
Cytosolic Thyroid Hormone-Binding Protein
Cthbp
Opa-Interacting Protein 3
Oip-3
Pyruvate Kinase 2/3
Pyruvate Kinase Muscle Isozyme
Threonine-Protein Kinase Pkm2
Thyroid Hormone-Binding Protein 1
Thbp1
Tumor M2-Pk
Tyrosine-Protein Kinase Pkm2
P58
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Cellular Localisation Isoform M2: Cytoplasm
Nucleus
Translocates To The Nucleus In Response To Various Signals
Such As Egf Receptor Activation Or Apoptotic Stimuli
Nuclear Translocation Is Promoted By Acetylation By Ep300
Deacetylation By Sirt6 Promotes Its Nuclear Export In A Process Dependent Of Xpo4
Thereby Suppressing Its Ability To Activate Transcription And Promote Tumorigenesis
Isoform M1: Cytoplasm
Alternative Antibody Names Anti-Pyruvate Kinase Pkm antibody
Anti-Cytosolic Thyroid Hormone-Binding Protein antibody
Anti-Cthbp antibody
Anti-Opa-Interacting Protein 3 antibody
Anti-Oip-3 antibody
Anti-Pyruvate Kinase 2/3 antibody
Anti-Pyruvate Kinase Muscle Isozyme antibody
Anti-Threonine-Protein Kinase Pkm2 antibody
Anti-Thyroid Hormone-Binding Protein 1 antibody
Anti-Thbp1 antibody
Anti-Tumor M2-Pk antibody
Anti-Tyrosine-Protein Kinase Pkm2 antibody
Anti-P58 antibody
Anti-PKM antibody
Anti-OIP3 antibody
Anti-PK2 antibody
Anti-PK3 antibody
Anti-PKM2 antibody

Information sourced from Uniprot.org

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