| Host: |
Goat |
| Applications: |
Pep-ELISA/IF/IHC/FC |
| Reactivity: |
Human/Mouse/Rat/Dog/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-PIWI/HIWI (Internal) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-4-6µg/mlIF-Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and HeLa cells. 10µg/mlELISA-antibody detection limit dilution 1:64000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
PIWIL1 |
| Gene ID: |
9271 |
| Uniprot ID: |
PIWL1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_004755.2; NP_001177900.1 |
| Specificity: |
This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1). |
| Immunogen Sequence: |
SNRKDKYDAIKKY |
| Post Translational Modifications | Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in late spermatids, leading to its degradation. Ubiquitination only takes place following piRNA-binding in adult testis. Ubiquitination and degradation in late spermatogenesis by APC/C is probably required to release RNF8 from the cytoplasm and promote histone to protamine exchange by RNF8. |
| Function | Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis.RNF8 being released upon ubiquitination and degradation of PIWIL1. Isoform 3: May be a negative developmental regulator. |
| Protein Name | Piwi-Like Protein 1 |
| Database Links | Reactome: R-HSA-5601884 |
| Cellular Localisation | CytoplasmComponent Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During MeiosisAlso Present In Chromatoid Body |
| Alternative Antibody Names | Anti-Piwi-Like Protein 1 antibodyAnti-PIWIL1 antibodyAnti-HIWI antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
