Anti-PISD antibody (1-375) (STJ116249)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PISD (1-375) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PISD |
| Gene ID | 23761 |
| Uniprot ID | PISD_HUMAN |
| Immunogen Region | 1-375 |
| Immunogen Sequence | MMCQSEARQGPELRAAKWLH FPQLALRRRLGQLSCMSRPA LKLRSWPLTVLYYLLPFGAL RPLSRVGWRPVSRVALYKSV PTRLLSRAWGRLNQVELPHW LRRPVYSLYIWTFGVNMKEA AVEDLHHYRNLSEFFRRKLK PQARPVCGLHSVISPSDGRI LNFGQVKNCEVEQVKGVTYS LESFLGPRMCTEDLPFPPAA SCDSFKNQLVTREGNELYHC VIYLAPGDYHCFHSPTDWT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human PISD (NP_055153.1). |
Additional Info
| Post Translational Modifications | Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase. |
| Function | Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane. |
| Protein Name | Phosphatidylserine Decarboxylase Proenzyme - Mitochondrial Cleaved Into - Phosphatidylserine Decarboxylase Beta Chain - Phosphatidylserine Decarboxylase Alpha Chain |
| Database Links | Reactome: R-HSA-1483213 |
| Cellular Localisation | Phosphatidylserine Decarboxylase Beta Chain: Mitochondrion Inner MembraneSingle-Pass Membrane ProteinIntermembrane SidePhosphatidylserine Decarboxylase Alpha Chain: Mitochondrion Inner MembranePeripheral Membrane ProteinCytoplasmAnchored To The Mitochondrial Inner Membrane Through Its Interaction With The Integral Membrane Beta ChainIsoform 1: Mitochondrion Inner MembraneIsoform 2: Mitochondrion Inner MembraneLipid DropletPredominantly Localizes To Lipid Droplets In Lipid-Replete ConditionsAnd To Mitochondria In Lipid-Deplete Conditions |
| Alternative Antibody Names | Anti-Phosphatidylserine Decarboxylase Proenzyme - Mitochondrial Cleaved Into - Phosphatidylserine Decarboxylase Beta Chain - Phosphatidylserine Decarboxylase Alpha Chain antibodyAnti-PISD antibody |
Information sourced from Uniprot.org