| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-PIP5K1A (231-500) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIP5K1A |
| Gene ID: | 8394 |
| Uniprot ID: | PI51A_HUMAN |
| Immunogen Region: | 231-500 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1). |
| Immunogen Sequence: | MNNLLPRSVKMHIKYDLKGS TYKRRASQKEREKPLPTFKD LDFLQDIPDGLFLDADMYNA LCKTLQRDCLVLQSFKIMDY SLLMSIHNIDHAQREPLSSE TQYSVDTRRPAPQKALYSTA MESIQGEARRGGTMETDDHM GGIPARNSKGERLLLYIGII DILQSYRFVKKLEHSWKALV HDGDTVSVHRPGFYAERFQR FMCNTVFKKIPCVHLGRPDV LPQTPPLEEISEGSPIPDP |
| Tissue Specificity | Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver. |
| Function | Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility. PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis. Can also use phosphatidylinositol (PtdIns) as substrate in vitro. Together with PIP5K1C, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps. Promotes particle ingestion by activating the WAS GTPase-binding protein that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, IP3 and DAG, that will mobilize internal calcium and drive keratinocyte differentiation. Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes. Together with PIP5K1C has a role during embryogenesis. Independently of its catalytic activity, is required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of the small GTPase RAC1 to the plasma membrane. Also functions in the nucleus where it acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. |
| Protein Name | Phosphatidylinositol 4-Phosphate 5-Kinase Type-1 AlphaPip5k1-AlphaPtdins(4p-5-Kinase 1 Alpha68 Kda Type I Phosphatidylinositol 4-Phosphate 5-Kinase AlphaPhosphatidylinositol 4-Phosphate 5-Kinase Type I AlphaPip5kialpha |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneCytoplasmNucleusNucleus SpeckleCell ProjectionRuffleLamellipodiumColocalizes With Rac1 At Actin-Rich Membrane RufflesLocalizes To Nuclear Speckles And Associates With Tut1 To Regulate Polyadenylation Of Selected Mrnas |
| Alternative Antibody Names | Anti-Phosphatidylinositol 4-Phosphate 5-Kinase Type-1 Alpha antibodyAnti-Pip5k1-Alpha antibodyAnti-Ptdins(4p-5-Kinase 1 Alpha antibodyAnti-68 Kda Type I Phosphatidylinositol 4-Phosphate 5-Kinase Alpha antibodyAnti-Phosphatidylinositol 4-Phosphate 5-Kinase Type I Alpha antibodyAnti-Pip5kialpha antibodyAnti-PIP5K1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit polyclonal antibody (STJ11100072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89142/357592/STJ11100072_1__02481.1713121730.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit polyclonal antibody (STJ11100072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit polyclonal antibody (STJ119998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98783/382833/STJ119998_1__70285.1713151340.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit polyclonal antibody (STJ119998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
