Anti-PIN4 antibody [R06-8I5] (STJA0034726)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human PIN4
General Information
| Short Description | Rabbit monoclonal anti-PIN4 for use in WB and IP in Human, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human/Rat/Hamster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R06-8I5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PIN4 |
| Gene ID | 5303 |
| Uniprot ID | PIN4_HUMAN |
| Immunogen | A synthetic peptide of human PIN4 |
Additional Info
| Tissue Specificity | Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed. |
| Post Translational Modifications | Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus. Isoform 2 is sumoylated with SUMO2 and SUMO3. |
| Function | Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Isoform 2 binds to double-stranded DNA. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 4Parvulin-14Par14Hpar14Parvulin-17Par17Hpar17Peptidyl-Prolyl Cis-Trans Isomerase Pin4Ppiase Pin4Peptidyl-Prolyl Cis/Trans Isomerase EpvhHepvhRotamase Pin4 |
| Database Links | |
| Cellular Localisation | Isoform 1: NucleusNucleolusCytoplasmCytoskeletonSpindleColocalizes In The Nucleolus During Interphase And On The Spindle Apparatus During Mitosis With Npm1Isoform 2: MitochondrionMitochondrion MatrixImported In A Time- And Membrane Potential-Dependent Manner To The Mitochondrial MatrixBut Without Concomitant Processing Of The ProteinDirected To Mitochondria By A Novel N-Terminal Domain That Functions As A Non-Cleavable Mitochondrial Targeting Peptide |
| Alternative Antibody Names | Anti-Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 4 antibodyAnti-Parvulin-14 antibodyAnti-Par14 antibodyAnti-Hpar14 antibodyAnti-Parvulin-17 antibodyAnti-Par17 antibodyAnti-Hpar17 antibodyAnti-Peptidyl-Prolyl Cis-Trans Isomerase Pin4 antibodyAnti-Ppiase Pin4 antibodyAnti-Peptidyl-Prolyl Cis/Trans Isomerase Epvh antibodyAnti-Hepvh antibodyAnti-Rotamase Pin4 antibodyAnti-PIN4 antibody |
Information sourced from Uniprot.org